ABSTRACT
BACKGROUND: The course of infectious bone nonunion is often difficult to heal and has a high risk of recurrence, which brings great trouble to patients’ lives. With the continuous research on the induced membrane technique, the advantages of the treatment of infectious nonunion with the induced membrane technique have gradually emerged and become a new research direction of medical work. OBJECTIVE: To explore the action mechanism of induced membrane technology in the treatment of infectious nonunion. METHODS: The relevant domestic and foreign literatures from 2013 to 2019 of PubMed, MEDLINE, Wanfang, CNKI, VIP, and Ubiquitous Chinese Biomedical Literature Database were retrieved. The key words were “induced membrane technique, infectious nonunion, induced membrane, growth factor” in Chinese and English. RESULTS AND CONCLUSION: The biological structure and function of the induced membrane are similar to that of the periosteum. It can induce the formation of osteoblasts and promote the growth of bone tissue. The induced membrane can secrete many kinds of growth factors, such as vascular endothelial growth factor, bone morphogenetic protein 2 and transforming growth factor β1. The application of induced membrane technology in clinic, especially in the field of fracture, has achieved remarkable results, which provides a new choice for the treatment of infectious nonunion. Exploring the most suitable stuffing material and bone grafting time and the dynamic ratio of the two have become the breakthrough point and challenge of this technology.
ABSTRACT
OBJECTIVE@#To construct and obtain ideal protein delivery vectors by researching the delivery efficiency and cytotoxicity to Hela cells using mPEG-PLGA-BSA-FITC-NPs.@*METHOD@#The mPEG-PLGA nanoparticle was obtained through surface modification of PLGA with PEG, and deliver BSA-FITC into Hela cells in vitro. The positive cells were counted by Laser scanning confocal microscopy and the survival rate of Hela cells was calculated by MTT assay at different time points.@*RESULT@#mPEG-PLGA-BSA-FITC-NPs shows the classic nanometer size, and the encapsulation efficiency reached 51. 2%. At the same time, the nanoparticles possess characteristics of slow release. By optimizing the delivery conditions, the highest efficiency of mPEG-PLGA-BSA-FITC-NPs was above 65.2%, and the cellular viability was about 85.7%.@*CONCLUSION@#mPEG-PLGA-BSA-FITC-NPs nanoparticles can successfully carry the target protein into cells as safe and effective as novel delivery materials of protein in vitro, and has shown slow release characteristics. The mPEG-PLGA-BSA-FITC-NPs provide ideal delivery vector for future application in clinical treatment of disease using nano-materials.
Subject(s)
Humans , Drug Carriers , Fluorescein-5-isothiocyanate , HeLa Cells , Nanoparticles , Particle Size , Polyesters , Polyethylene Glycols , Serum Albumin, BovineABSTRACT
Objective To explore the clinical effects of atlantoaxial pedicle screw instrumentation in treatment of intractable atlantoaxial dislocation in children. Methods A total of 7 patients with intractable atlantoaxial dislocations were treated with aflantoaxial pedicle screw instrumentation plus atlantoaxial bone grafting from June 2002 to January 2001. Results The dislocation in all patients reached complete reduction, with no complications. All patients were followed up for average 10 months (8-14 months). Radiographs showed successful bone fusion in all patients. Conclusion Atlantoaxial pedicle screw fixation and fusion is an effective method for treatment of intractable atlantoaxial dislocation in chil dren.